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OGDHL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OGDHL Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G126292-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OGDHL (NM_001143997), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1290 | OGDC-E1-like | OGDHL
Origin Species
Human
NCBI GenBank
NM_018245
SwissProt
Q9ULD0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFINDVEQCQ WIRQKFETPG VMQFSSEEKR TLLARLVRSM RFEDFLARKW SSEKRFGLEG
61CEVMIPALKT IIDKSSEMGI ENVILGMPHR GRLNVLANVI RKDLEQIFCQ FDPKLEAADE
121GSGDVKYHLG MYHERINRVT NRNITLSLVA NPSHLEAVDP VVQGKTKAEQ FYRGDAQGKK
181VMSILVHGDA AFAGQGVVYE TFHLSDLPSY TTNGTVHVVV NNQIGFTTDP RMARSSPYPT
241DVARVVNAPI FHVNADDPEA VIYVCSVAAE WRNTFNKDVV VDLVCYRRRG HNEMDEPMFT
301QPLMYKQIHR QVPVLKKYAD KLIAEGTVTL QEFEEEIAKY DRICEEAYGR SKDKKILHIK
361HWLDSPWPGF FNVDGEPKSM TCPATGIPED MLTHIGSVAS SVPLEDFKIH TGLSRILRGR
421ADMTKNRTVD WALAEYMAFG SLLKEGIHVR LSGQDVERGT FSHRHHVLHD QEVDRRTCVP
481MNHLWPDQAP YTVCNSSLSE YGVLGFELGY AMASPNALVL WEAQFGDFHN TAQCIIDQFI
541STGQAKWVRH NGIVLLLPHG MEGMGPEHSS ARPERFLQMS NDDSDAYPAF TKDFEVSQLY
601DCNWIVVNCS TPANYFHVLR RQILLPFRKP LIIFTPKSLL RHPEAKSSFD QMVSGTSFQR
661VIPEDGAAAR APEQVQRLIF CTGKVYYDLV KERSSQDLEE KVAITRLEQI SPFPFDLIKQ
721EAEKYPGAEL AWCQEEHKNM GYYDYISPRF MTILRRARPI WYVGRDPAAA PATGNRNTHL
781VSLKKFLDTA FNLQAFEGKT F
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

801 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.