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ODF2L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ODF2L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ODF2L antibodies →
$725.00each
Catalog Number: LS-G111040-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ODF2L (NM_020729), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ977L11.1 | ODF2L | RP5-977L11.1 | KIAA1229
Origin Species
Human
NCBI GenBank
AB033055
SwissProt
Q9ULJ1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKAVNDGSH SEELFCHLKT ISEKEDLPRC TSESHLSCLK QDILNEKTEL EATLKEAELV
61THSVELLLPL FKDTIEKINF ENANLSALNL KISEQKEILI KELDTFKSVK LALEHLLRKR
121DYKQTGDNLS SMLLENLTDN ESENTNLKKK VFEKEAHIQE LSCLFQSEKA NTLKANRFSQ
181SVKVVHERLQ IQIHKREAEN DKLKEYVKSL ETKIAKWNLQ SRMNKNEAIV MKEASRQKTV
241ALKKASKVYK QRLDHFTGAI EKLTSQIRDQ EAKLSETISA SNAWKSHYEK IVIEKTELEV
301QIETMKKQII NLLEDLKKME DHGKNSCEEI LRKVHSIEYE NETLNLENTK LKTTLAALKD
361EVVSVENELS ELQEVEKKQK TLIEMYKTQV QKLQEAAEIV KSRCENLLHK NNQITKTKNK
421NVEKMRGQME SHLKELERVC DSLTAAERRL HECQESLQCC KGKCADQEHT IRELQGQILK
481QWEEYSVLAW VDGNHNLLTK LSLEEENCLI QLKCENLQQK LEQMDAENKE LEKKLANQEE
541CLKHSNLKFK EKSAEYTALA RQLEAALEEG RQKVAEEIEK MSSRESALQI KILDLETELR
601KKNEEQNQLV CKMNSDPETP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

620 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.