Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
OCSP / TSPAN10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109122-100
Catalog Number: LS-G109122
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TSPAN10 (NM_031945), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- OCSP | Tetraspanin-10 | Oculospanin | Tetraspanin 10 | Tspan-10 | TSPAN10
- Origin Species
- Human
- NCBI GenBank
- NM_031945
- SwissProt
- Q9H1Z9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEEGERSPLL SQETAGQKPL SVHRPPTSGC LGPVPREDQA EAWGCSCCPP ETKHQALSGT61PKKGPAPSLS PGSSCVKYLI FLSNFPFSLL GLLALAIGLW GLAVKGSLGS DLGGPLPTDP121MLGLALGGLV VSAASLAGCL GALCENTCLL RGFSGGILAF LVLEAVAGAL VVALWGPLQD181SLEHTLRVAI AHYQDDPDLR FLLDQVQLGL RCCGAASYQD WQQNLYFNCS SPGVQACSLP241ASCCIDPRED GASVNDQCGF GVLRLDADAA QRVVYLEGCG PPLRRWLRAN LAASGGYAIA301VVLLQGAELL LAARLLGALA ARSGAAYGPG ARGEDRAGPQ SPSPGAPPAA KPARGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
355 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.