Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
OASIS / CREB3L1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102144-100
Catalog Number: LS-G102144
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CREB3L1 (NM_052854), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BBF-2 homolog | CREB3L1 | OASIS
- Origin Species
- Human
- NCBI GenBank
- NM_052854
- SwissProt
- Q96BA8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDAVLEPFPA DRLFPGSSFL DLGDLNESDF LNNAHFPEHL DHFTENMEDF SNDLFSSFFD61DPVLDEKSPL LDMELDSPTP GIQAEHSYSL SGDSAPQSPL VPIKMEDTTQ DAEHGAWALG121HKLCSIMVKQ EQSPELPVDP LAAPSAMAAA AAMATTPLLG LSPLSRLPIP HQAPGEMTQL181PVIKAEPLEV NQFLKVTPED LVQMPPTPPS SHGSDSDGSQ SPRSLPPSSP VRPMARSSTA241ISTSPLLTAP HKLQGTSGPL LLTEEEKRTL IAEGYPIPTK LPLTKAEEKA LKRVRRKIKN301KISAQESRRK KKEYVECLEK KVETFTSENN ELWKKVETLE NANRTLLQQL QKLQTLVTNK361ISRPYKMAAT QTGTCLMVAA LCFVLVLGSL VPCLPEFSSG SQTVKEDPLA ADGVYTASQM421PSRSLLFYDD GAGLWEDGRS TLLPMEPPDG WEINPGGPAE QRPRDHLQHD HLDSTHETTK481YLSEAWPKDG GNGTSPDFSH SKEWFHDRDL GPNTTIKLSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
519 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.