LSBio
NYX Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NYX Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NYX antibodies →
$725.00each
Catalog Number: LS-G110320-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NYX (NM_022567), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CSNB1 | CSNB1A | CSNB4 | CLRP | NBM1 | NYX | Nyctalopin | Leucine-rich repeat protein
Origin Species
Human
NCBI GenBank
NM_022567
SwissProt
Q9GZU5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
49.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKGRGMLVLL LHAVVLGLPS AWAVGACARA CPAACACSTV ERGCSVRCDR AGLLRVPAEL
61PCEAVSIDLD RNGLRFLGER AFGTLPSLRR LSLRHNNLSF ITPGAFKGLP RLAELRLAHN
121GDLRYLHART FAALSRLRRL DLAACRLFSV PERLLAELPA LRELAAFDNL FRRVPGALRG
181LANLTHAHLE RGRIEAVASS SLQGLRRLRS LSLQANRVRA VHAGAFGDCG VLEHLLLNDN
241LLAELPADAF RGLRRLRTLN LGGNALDRVA RAWFADLAEL ELLYLDRNSI AFVEEGAFQN
301LSGLLALHLN GNRLTVLAWV AFQPGFFLGR LFLFRNPWCC DCRLEWLRDW MEGSGRVTDV
361PCASPGSVAG LDLSQVTFGR SSDGLCVDPE ELNLTTSSPG PSPEPAATTV SRFSSLLSKL
421LAPRVPVEEA ANTTGGLANA SLSDSLSSRG VGGAGRQPWF LLASCLLPSV AQHVVFGLQM
481D
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

481 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.