Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NXPE3 / FAM55C Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125291-100
Catalog Number: LS-G125291
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM55C(NXPE3) (NM_001134456), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001134456
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MWTNFFKLRL FCCLLAVLMV VVLVINVTQV EYLDHETVSA TFIDSSGQFV SSQVTGISRN61PYCGYDQQTL SSQERMEEDS LLAALHRQVP DVGPVPFVKS TDPSSSYFVI LNSAAFFKVG121SQLEVLVHVQ DFQRKPKKYG GDYLQARIHS LKLQAGAVGR VVDYQNGFYK VFFTLLWPGK181VKVSVSLVHP SEGIRVLQRL QEDKPDRVYF KSLFRSGRIS ETTECNVCLP GNLPLCNFTD241LYTGEPWFCF KPKKLPCSSR ITHFKGGYLK GLLTAAESAF FQSGVNIKMP VNSSGPDWVT301VIPRRIKETN SLELSQGSGT FPSGYYYKDQ WRPRKFKMRQ FNDPDNITEC LQRKVVHLFG361DSTIRQWFEY LTTFVPDLVE FNLGSPKNVG PFLAVDQKHN ILLKYRCHGP PIRFTTVFSN421ELHYVANELN GIVGGKNTVV AIAVWSHFST FPLEVYIRRL RNIRRAVVRL LDRSPKTVVV481IRTANAQELG PEVSLFNSDW YNFQLDTILR RMFSGVGVYL VDAWEMTLAH YLPHKLHPDE541VIVKNQLDMF LSFVCPLETTRTRPLEQKLISEEDLAANDILDYKDDDDKV
559 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.