Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NXP2 / MORC3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113287-100
Catalog Number: LS-G113287
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MORC3 (NM_015358), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0136 | MORC3 | Nuclear matrix protein NXP2 | NXP2 | ZCW5 | ZCWCC3
- Origin Species
- Human
- NCBI GenBank
- D50926
- SwissProt
- Q14149
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 106.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAQPPRGIR LSALCPKFLH TNSTSHTWPF SAVAELIDNA YDPDVNAKQI WIDKTVINDH61ICLTFTDNGN GMTSDKLHKM LSFGFSDKVT MNGHVPVGLY GNGFKSGSMR LGKDAIVFTK121NGESMSVGLL SQTYLEVIKA EHVVVPIVAF NKHRQMINLA ESKASLAAIL EHSLFSTEQK181LLAELDAIIG KKGTRIIIWN LRSYKNATEF DFEKDKYDIR IPEDLDEITG KKGYKKQERM241DQIAPESDYS LRAYCSILYL KPRMQIILRG QKVKTQLVSK SLAYIERDVY RPKFLSKTVR301ITFGFNCRNK DHYGIMMYHR NRLIKAYEKV GCQLRANNMG VGVVGIIECN FLKPTHNKQD361FDYTNEYRLT ITALGEKLND YWNEMKVKKN TEYPLNLPVE DIQKRPDQTW VQCDACLKWR421KLPDGMDQLP EKWYCSNNPD PQFRNCEVPE EPEDEDLVHP TYEKTYKKTN KEKFRIRQPE481MIPRINAELL FRPTALSTPS FSSPKESVPR RHLSEGTNSY ATRLLNNHQV PPQSEPESNS541LKRRLSTRSS ILNAKNRRLS SQFENSVYKG DDDDGDVIIL EENSTPKPAV DHDIDMKSEQ601SHVEQGGVQV EFVGDSEPCG QTGSTSTSSS RCDQGNTAAT QTEVPSLVVK KEETVEDEID661VRNDAVILPS CVEAEAKIHE TQETTDKSAD DAGCQLQELR NQLLLVTEEK ENYKRQCHMF721TDQIKVLQQR ILEMNDKYVK KETCHQSTET DAVFLLESIN GKSESPDHMV SQYQQALEEI781ERLKKQCSAL QHVKAECSQC SNNESKSEMD EMAVQLDDVF RQLDKCSIER DQYKSEVELL841EMEKSQIRSQ CEELKTEVEQ LKSTNQQTAT DVSTSSNIEE SVNHMDGESL KLRSLRVNVG901QLLAMIVPDL DLQQVNYDVD VVDEILGQVV EQMSEISSTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
939 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.