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NXF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NXF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110430-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NXF3 (NM_022052), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NXF3 | Nuclear RNA export factor 3 | TAPL-3 | TAP-like protein 3 | TAPL3
Origin Species
Human
NCBI GenBank
NM_022052
SwissProt
Q9H4D5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLPSGHTTG HTDQVVQRRA RCWDIYQRRF SSRSEPVNPG MHSSSHQQQD GDAAMHGAHM
61DSPVRYTPYT ISPYNRKGSF RKQDQTHVNM EREQKPPERR MEGNMPDGTL GSWFKITVPF
121GIKYNEKWLL NLIQNECSVP FVPVEFHYEN MHASFFVENA SIAYALKNVS GKIWDEDNEK
181ISIFVNPAGI PHFVHRELKS EKVEQIKLAM NQQCDVSQEA LDIQRLPFYP DMVNRDTKMA
241SNPRKCMAAS LDVHEENIPT VMSAGEMDKW KGIEPGEKCA DRSPVCTTFS DTSSNINSIL
301ELFPKLLCLD GQQSPRATLC GTEAHKRLPT CKGSFFGSEM LKNLVLQFLQ QYYLIYDSGD
361RQGLLSAYHD EACFSLSIPF NPEDSAPSSF CKFFKDSRNI KILKDPYLRG ELLKHTKLDI
421VDSLSALPKT QHDLSSFLVD MWYQTEWMLC FSVNGVFKEV EGQSQGSVLA FTRTFIATPG
481SSSSLCIVND KLFVRDTSHQ GTQSALFTLV PTAFSSSVPA FSQEQQKMLP S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

531 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.