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NXF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NXF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112193-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NXF2 (NM_017809), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 39 | CT39 | NXF2 | TAPL2 | TAPL-2 | T-complex protein 11 homolog | TAP-like protein 2 | Nuclear RNA export factor 2
Origin Species
Human
NCBI GenBank
NM_022053
SwissProt
Q9GZY0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCSTLKKCGT YRTEVAECHD HGSTFQGRKK GGSSFRDNFD KRSCHYEHGG YERPPSHCQE
61NDGSVEMRDV HKDQQLRHTP YSIRCERRMK WHSEDEIRIT TWRNRKPPER KMSQNTQDGY
121TRNWFKVTIP YGIKYDKAWL MNSIQSHCSD RFTPVDFHYV RNRACFFVQD ASAASALKDV
181SYKIYDDENQ KICIFVNHST APYSVKNKLK PGQMEMLKLT MNKRYNVSQQ ALDLQNLRFD
241PDLMGRDIDI ILNRRNCMAA TLKIIERNFP ELLSLNLCNN KLYQLDGLSD ITEKAPKVKT
301LNLSKNKLES AWELGKVKGL KLEELWLEGN PLCSTFSDQS AYVSAIRDCF PKLLRLDGRE
361LSAPVIVDID SSETMKPCKE NFTGSETLKH LVLQFLQQYY SIYDSGDRQG LLGAYHDEAC
421FSLAIPFDPK DSAPSSLCKY FEDSRNMKTL KDPYLKGELL RRTKRDIVDS LSALPKTQHD
481LSSILVDVWC QTERMLCFSV NGVFKEVEGQ SQGSVLAFTR TFIATPGSSS SLCIVNDELF
541VRDASPQETQ SAFSIPVSTL SSSSEPSLSQ EQQEMVQAFS AQSGMKLEWS QKCLQDNEWN
601YTRAGQAFTM LQTEGKIPAE AFKQIS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

626 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.