Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NWD2 / KIAA1239 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G126461-100
Catalog Number: LS-G126461
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1239(NWD2) (NM_001144990), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1239
- Origin Species
- Human
- NCBI GenBank
- AB033065
- SwissProt
- Q9ULI1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 197.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWPAGAGTKL PCPRDSALRR AAFSGNLTAL PSHLVPAGRS VRVFISANPE DTGAERQALR61ENVYPKLREF CRENYGLEFQ VIDLYWGVEE DEWDSPELQK TRMKLLENCL KTSAGPCFVG121LLGEKYGNIR IPGEVEASEF EMILDAAIEA KLETKLLEEW YCRDENSVPA AYYLRPKSEM181LRSNRNAMQP STNAENEKTW QEISDEIKKI FKAAVKLLHE KGKMKHSQAK RYLFSAIEDE241FDFALGKQTP AFLKKCVCYI RKIANIERFV KIPEMGKYMD ITGTEPRIIR DPEAQEKLIK301LRDEFIPTIV ASSNLRVYTS VTHCDMKLGY SQEIENHYIE GLGKQFYEDM IDIIQATIQQ361NFDTETDTLY DEILQHSSLC KTYASFYEYK CESLNIVHNY ILPSKAGHIN PLIIYGGPCT421GKTLLLAEVA KKAYGWLHED TGPESDPVVI VRFLGTTDMS SDLRTLLLSV CEQLAVNYRC481LVQSYPKKIH DLCDLFINLL NESSLQRPLV IIFDALEQLS ENDDARKLWW LPAHLPRFVR541IVLSTLPNKH GILQKLRCLI HEEDNYIELI PRDRKMCSQV LKHQLLRVKR KVTSGQQIYV601NNALSKCTLP MFVNLTFREV RHWRSHKDVD ESSLSVTVHE SIEQLFWSLE KKCGQKLVSR661ALGYITMAKM GLSEMELEDV LALDNSVMSE LKENTRPSNP LRVPYLYIAR LKEGLSGYLI721ERHVKNVTLL VWANRHLQLI AQKLYLQDDN DLREMHTILA DYFLGVWSGG RRKAFCLEDP781YLNGCLDLEN RSLLEEEKHF MEQASFDRQA PDQPWVFQCN PLEPDIFFVN HRKMSELLYH841LTRCGKTDDL LYGIIMNFSW LYTMIKIGQF DKVLSDIELA YNYSQEKELK FLANTLRSIK901NKVTAFPGSL SAELQQRLLP VVSSLPKLRH LLLECDKDGP KYCSIVPLHS SMDVTYSPER961LPLSSSHLHV TEILPTCNPS TVLTALENGS ISTWDVETRQ LLRQITTAQS VILGMKLTSD1021EKYLVVATTN NTLLIYDNVN SCLLSEVEIK GTKHGSSATY INGFTLSANH ALAWLEASKD1081VTVIDLLYGW PLYQFHCWYE VTCVQCSLDG LYAFCGQYLN TTTIFHLGSG EKLCTVTSEF1141SGGFVKFLLI LDTAQEMVMV DSEGSLSVWN TEDISSPQLT DDFDCRREDS EVVSIELSED1201QSAVLICKAL SIELLDTGLW KVAEKFRAKH NERFISAVLS KNGDCIIATM ENTSAVFFWR1261RDTGQCMASL QEISGSIVKL VKSSHHNMLL SLSTSGVLSI WDIDIITAMS NIDKTGKPIQ1321SLLLPARGEI IYSLDGSDCV HKWNFSSGFI EAVFKHEGIV EHCVLTSTGD IMVTSDDKSS1381QYVWHTSSGE NLFRINGQRI SQLLITHNDQ FVVSLCEENA SRVWRLATGH RVCNILTTLQ1441NAFITSANTF VVGMTKSKVL AVSLWTGSIT KKFCCEDGTT IVNFKLIPDC PDIIVFITSA1501ETVNIWSLTD EVICRRVQLP NNFLKNLEDF EISPNGKLGI IARGDENINV LDLYSGKLRV1561VHASGIIWRQ RLSRDGRYLV YICFRNGEEE DENGAIFSLI VMRLADGKNI GACSLYKTPT1621FLALSQRHLN IIVGFDDGSI GIYTVVDRVD AALKIKIATS NSRQIFNNAT HTSRPKCNSY1681CFKISVDCLW RESTEVFARD SPITVSDSTE SNEATPSKKH NSCYERVCSA LEARGHSYAP1741DNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1742 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.