Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NUTM2G / FAM22G Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G130205-100
Catalog Number: LS-G130205
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NUTM2G (NM_001170741), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FAM22G | Protein FAM22G
- Origin Species
- Human
- NCBI GenBank
- NM_001045477
- SwissProt
- Q5VZR2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASNGAYPVL GPGVTVNPGT SLSVFTALPF ATPSPGPTHR PPLVTAVVPP AGPLVLSAFP61STPLVAGQDG RGPSGAGASN VFVQMRTEVG PVKPPQAQTL ILTQAPLVWQ APGTLCGGVM121CPPPLLLAAA PGVPVTSAQV VGGTQACEGG WSHGLPLPPP PPAAQVAPIV SPGNAGPWPQ181GAHGEGSLAP SQAKARPDDS CKPKSVYENF RLWQHYKPLA RRHLPQSPDT EALSCFLIPV241LRSLARRKPT MTLEEGLWRA MREWQHTSNF DRMIFYEMAA KFLEFEAEEE MQIQKSQWMK301GPQSLPPPAP PRLEPRGPPA PEVVKQPVYL PSKDGPKAPT ACLPPPRPQR PAETKAHLPP361PRPPRPAETK VPEEIPPEVV QEYVDIMEEL LGSHPGDTGE PEGQREKGKV EQPQEEDGMT421SDPGLLSYID KLCSQEDFVT KVEAVIHPRF LEELLSPDPQ MDFLALSQEL EQEEGLTLAQ481LVEKRLLSLK EKGCGRAAPR HGTARLDSSP SEFAAGQEAA REVPDPQQRV SVETSPPQTA541AQDPQGQGRV RTGMARSEDP AVLLGCQDSP RLKAVRPTSP PQDHRPTCPG LGTKDALGLP601GESPVKESHG LAKGSSEETE LPGMVYVVGS HHRLRPWRLS QSPVPSSGLL SPGGRGPQGA661LQSPSAQKRG LSPSPSPASK SKKRPLFGSP SPAEKTPHPG PGLRVSGEQS LAWGLGGPSQ721SQKRKGDPLA SRRKKKRHCS QTRTRPLEQKLISEEDLAANDILDYKDDDDKV
741 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.