Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NUTM2F / FAM22F Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112377-100
Catalog Number: LS-G112377
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NUTM2F (NM_017561), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_017561
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASNGAYPVL GPGVTVNPGT SLSVFTALPF ATPAPGPAHR PPLVTAVVPP AGPLVLSAFP61STPLVAGQDG RGPSGAGASN VFVQMRTEVG PVKPPQAQTL ILTQAPLVWQ APGTLCGGVM121CPPPLLLAAA PGVPVTSAQV VGGTQACEGG WSHGLPLPPP PPAAQVAPIV SPGNARPWPQ181GAHGEGSLAP SQAKARPDDS CKPKSVYENF RLWQHYKPLA RRHLPQSPDT EALSCFLIPV241LRSLARRKPT MTLEEGLWQA MREWQHTSNF DRMIFYEMAE KFLEFEAEEE MQIQKSQWMK301GPQSLPPPAP PRLEPRGPPA PEVVKQPVYL PSKDGPKAPT ACLPPPRPQR PAETKAHLPP361PRPQRPAETN AHLPPPRPQR PAETKVPEEI PPEVVQEYVD IMEELLGSHP GDTGEPEGQR421EKGKVEQPQE EDGITSDPGL LSYIDKLCSQ EDFVTKVEAV IHPRFLEELL SPDPQMDFLA481LSQELEQEEG LTLAQLVEKR LLSLKEKGCG RAAPRHGTAR LDSSPSEFAA GQEAAREVPD541PQQRVSVETS PPQTAAQDPQ GQGRVRTGMA RSEDPAVLLG CQDSPRLKAV RPTSPPQDHR601PTCPGLGTKD ALGLPGESPV KESHGLAKGS SEETELPGMV YVVGSHHRLR PWRLSQSPVP661SSGLLSPGGR GPQGALQSPS AQKRGLSPSP SPASKSKKRP LFGSPSPAEK TPHPGPGLRV721SGEQSLAWGL GGPSQSQKRK GDPLASRRKK KRHCSQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
756 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.