Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NUTM2D / FAM22D Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121453-100
Catalog Number: LS-G121453
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM22D (NM_001009610), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FAM22D | Protein FAM22D
- Origin Species
- Human
- NCBI GenBank
- NM_001009610
- SwissProt
- Q5VT03
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFQEPVYFQI FLFQLDSGAS GEPGHSLGLT LGFSHCGNCQ TAVVSAQPEG MASNGAYPAL61GPGVTANPGT SLSVFTALPF TTPAPGPAHG PLLVTAGAPP GGPLVLSNFP STPLVTEQDG121CGPSGAGASN VFVQMRTEVG PVKAAQAQTL VLTQTPLVWQ APGALCGGVV CPPPLLLAAA181PVVPVMAAQV VGGTQACEGG WSQGLPLPPP PPPAAQLPPI VSQGNAGPWP QGAHGEGSLA241SSQAKAPPDD SCNPRSVYEN FRLWQHYKPL ARRHLPQSPD TEALSCFLIP VLRSLARRKP301TMTLEEGLWR AMREWQHTSN FDRMIFYEMA EKFLEFEAEE EMQIQKSQWM KGPQCLPPPA361TPRLEPRGPP APEVVKQPVY LPSKAGPKAP TACLPPPRPQ RPVTKARRPP PRPHRRAETK421ARLPPPRPQR PAETKVPEEI PPEVVQEYVD IMEELLGPSL GATGEPEKQR EEGEVKQPQE481EDWTPPDPGL LSYTDKLCSQ KDFVTKVEAV IHPQFLEELL SPDPQMDFLA LSQELEQEEG541LTLAQGAPSD APGTDRCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
557 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.