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NUPL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NUPL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114703-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NUPL2 (NM_007342), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CG1 | H_RG271G13.9 | NLP_1 | Nucleoporin-like protein 2 | Nucleoporin-like protein 1 | Nucleoporin like 2 | NUP42 homolog | HCG1 | NLP-1 | Nucleoporin hCG1 | NUPL2
Origin Species
Human
NCBI GenBank
NM_007342
SwissProt
O15504

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
44.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MAICQFFLQG RCRFGDRCWN EHPGARGAGG GRQQPQQQPS GNNRRGWNTT SQRYSNVIQP
61SSFSKSTPWG GSRDQEKPYF SSFDSGASTN RKEGFGLSEN PFASLSPDEQ KDEKKLLEGI
121VKDMEVWESS GQWMFSVYSP VKKKPNISGF TDISPEELRL EYHNFLTSNN LQSYLNSVQR
181LINQWRNRVN ELKSLNISTK VALLSDVKDG VNQAAPAFGF GSSQAATFMS PGFPVNNSSS
241DNAQNFSFKT NSGFAAASSG SPAGFGSSPA FGAAASTSSG ISTSAPAFGF GKPEVTSAAS
301FSFKSPAASS FGSPGFSGLP ASLATGPVRA PVAPAFGGGS SVAGFGSPGS HSHTAFSKPS
361SDTFGNSSIS TSLSASSSIV ATDNVLFTPR DKLTVEELEQ FQSKKFTLGK IPLKPPPLEL
421LNV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

423 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.