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NUPL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NUPL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114144-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NUPL1(NUP58) (NM_014089), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0410 | Nucleoporin-like protein 1 | PRO2463 | NUPL1 | Nucleoporin like 1 | Nucleoporin p58/p45
Origin Species
Human
NCBI GenBank
AB007870
SwissProt
Q9BVL2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSTGFSFGSG TLGSTTVAAG GTSTGGVFSF GTGASSNPSV GLNFGNLGST STPATTSAPS
61SGFGTGLFGS KPATGFTLGG TNTGIATTIT TGLTLGTPAT TSAATTGFSL GFNKPAASAT
121PFALPITSTS ASGLTLSSAL TSTPAASTGF TLNNLGGTTA TTTTASTGLS LGGALAGLGG
181SLFQSTNTGT SGLGQNALGL TLGTTAATST AGNEGLGGID FSSSSDKKSD KTGTRPEDSK
241ALKDENLPPV ICQDVENLQK FVKEQKQVQE EISRMSSKAM LKVQEDIKAL KQLLSLAANG
301IQRNTLNIDK LKIETAQELK NAEIALRTQK TPPGLQHEYA APADYFRILV QQFEVQLQQY
361RQQIEELENH LATQANNSHI TPQDLSMAMQ KIYQTFVALA AQLQSIHENV KVLKEQYLGY
421RKMFLGDAVD VFETRRAEAK KWQNTPRVTT GPTPFSTMPN AAAVAMAATL TQQQQPATGP
481QPSLGVSFGT PFGSGIGTGL QSSGLGSSNL GGFGTSSGFG CSTTGASTFG FGTTNKPSGS
541LSAGFGSSST SGFNFSNPGI TASAGLTFGV SNPASAGFGT GGQLLQLKKP PAGNKRGKRT
601RTRPLEQKLI SEEDLAANDI LDYKDDDDKV

630 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.