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NUP54 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NUP54 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112458-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NUP54 (NM_017426), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
54 kDa nucleoporin | Nucleoporin 54kD | NUP54 | Nucleoporin 54kDa | Nucleoporin p54
Origin Species
Human
NCBI GenBank
NM_017426
SwissProt
Q7Z3B4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MAFNFGAPSG TSGTAAATAA PAGGFGGFGT TSTTAGSAFS FSAPTNTGTT GLFGGTQNKG
61FGFGTGFGTT TGTSTGLGTG LGTGLGFGGF NTQQQQQTTL GGLFSQPTQA PTQSNQLINT
121ASALSAPTLL GDERDAILAK WNQLQAFWGT GKGYFNNNIP PVEFTQENPF CRFKAVGYSC
181MPSNKDEDGL VVLVFNKKET EIRSQQQQLV ESLHKVLGGN QTLTVNVEGT KTLPDDQTEV
241VIYVVERSPN GTSRRVPATT LYAHFEQANI KTQLQQLGVT LSMTRTELSP AQIKQLLQNP
301PAGVDPIIWE QAKVDNPDSE KLIPVPMVGF KELLRRLKVQ DQMTKQHQTR LDIISEDISE
361LQKNQTTSVA KIAQYKRKLM DLSHRTLQVL IKQEIQRKSG YAIQADEEQL RVQLDTIQGE
421LNAPTQFKGR LNELMSQIRM QNHFGAVRSE ERYYIDADLL REIKQHLKQQ QEGLSHLISI
481IKDDLEDIKL VEHGLNETIH IRGGVFS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

507 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.