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NUP210L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NUP210L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NUP210L antibodies →
$725.00each
Catalog Number: LS-G102868-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NUP210L (NM_207308), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_207308

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
210.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTGCPASSRR RGFGLFFFLR LHRLLLLFLV LRGTLANKLN VPQVLLPFGR EPGRVPFLLE
61AQRGCYTWHS THHDAVTVEP LYENGTLCSQ KAVLIAESTQ PIRLSSIILA REIVTDHELR
121CDVKVDVINS IEIVSRAREL YVDDSPLELM VRALDAEGNT FSSLAGMMFE WSIAQDNESA
181REELSSKIRI LKYSEAEYAP PIYIAEMEKE EKQGDVILVS GIRTGAAVVK VRIHEPFYKK
241VAAALIRLLV LENIFLIPSH DIYLLVGTYI KYQVAKMVQG RVTEVKFPLE HYILELQDHR
301VALNGSHSEK VAILDDKTAM VTASQLGQTN LVFVHKNVHM RSVSGLPNCT IYVVEPGFLG
361FTVQPGNRWS LEVGQVYVIT VDVFDKSSTK VYISDNLRIT YDFPKEYFEE QLTTVNGSYH
421IVKALKDGVV VINASLTSII YQNKDIQPIK FLIKHQQEVK IYFPIMLTPK FLAFPHHPMG
481MLYRYKVQVE GGSGNFTWTS SNETVVIVTT KGVVTAGQVR GNSTVLARDV QNPFRYGEIK
541IHVLKLNKME LLPFHADVEI GQIIEIPIAM YHINKETKEA MAFTDCSHLS LDLNMDKQGV
601FTLLKEGIQR PGPMHCSSTH IAAKSLGHTL VTVSVNECDK YLESSATFAA YEPLKALNPV
661EVALVTWQSV KEMVFEGGPR PWILEPSRFF LELNAEKTEK IGIAQVWLPS KRKQNQYIYR
721IQCLDLGEQV LTFRIGNHPG VLNPSPAVEV LQVRFICAHP ASMSVTPVYK VPAGAQPCPL
781PQHNKWLIPV SRLRDTVLEL AVFDQHRRKF DNFSSLMLEW KSSNETLAHF EDYKSVEMVA
841KDDGSGQTRL HGHQILKVHQ IKGTVLIGVN FVGYSEKKSP KEISNLPRSV DVELLLVDDV
901TVVPENATIY NHPDVKETFS LVEGSGYFLV NSSEQGVVTI TYMEAESSVE LVPLHPGFFT
961LEVYDLCLAF LGPATAHLRV SDIQELELDL IDKVEIDKTV LVTVRVLGSS KRPFQNKYFR
1021NMELKLQLAS AIVTLTPMEQ QDEYSENYIL RATTIGQTTL VAIAKDKMGR KYTSTPRHIE
1081VFPPFRLLPE KMTLIPMNMM QVMSEGGPQP QSIVHFSISN QTVAVVNRRG QVTGKIVGTA
1141VVHGTIQTVN EDTGKVIVFS QDEVQIEVVQ LRAVRILAAA TRLITATKMP VYVMGVTSTQ
1201TPFSFSNANP GLTFHWSMSK RDVLDLVPRH SEVFLQLPVE HNFAMVVHTK AAGRTSIKVT
1261VHCMNSSSGQ FEGNLLELSD EVQILVFEKL QLFYPECQPE QILMPINSQL KLHTNREGAA
1321FVSSRVLKCF PNSSVIEEDG EGLLKAGSIA GTAVLEVTSI EPFGVNQTTI TGVQVAPVTY
1381LRVSSQPKLY TAQGRTLSAF PLGMSLTFTV QFYNSIGEKF HTHNTQLYLA LNRDDLLHIG
1441PGNKNYTYMA QAVNRGLTLV GLWDRRHPGM ADYIPVAVEH AIEPDTKLTF VGDIICFSTH
1501LVSQHGEPGI WMISANNILQ TDIVTGVGVA RSPGTAMIFH DIPGVVKTYR EVVVNASSRL
1561MLSYDLKTYL TNTLNSTVFK LFITTGRNGV NLKGFCTPNQ ALAITKVLLP ATLMLCHVQF
1621SNTLLDIPAS KVFQVHSDFS MEKGVYVCII KVRPQSEELL QALSVADTSV YGWATLVSER
1681SKNGMQRILI PFIPAFYINQ SELVLSHKQD IGEIRVLGVD RVLRKLEVIS SSPVLVVAGH
1741SHSPLTPGLA IYSVRVVNFT SFQQMASPVF INISCVLTSQ SEAVVVRAMK DKLGADHCED
1801SAILKRFTGS YQILLLTLFA VLASTASIFL AYNAFLNKIQ TVPVVYVPTL GTPQPGFFNS
1861TSSPPHFMSL QPPLAQSRLQ HWLWSIRH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1888 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.