Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NUP210 / gp210 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109653-100
Catalog Number: LS-G109653
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GP210(NUP210) (NM_024923), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- gp210 | KIAA0906 | Nucleoporin 210kDa | Nucleoporin Nup210 | Nuclear pore protein gp210 | NUP210 | POM210
- Origin Species
- Human
- NCBI GenBank
- NM_024923
- SwissProt
- Q8TEM1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 204.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAARGRGLLL LTLSVLLAAG PSAAAAKLNI PKVLLPFTRA TRVNFTLEAS EGCYRWLSTR61PEVASIEPLG LDEQQCSQKA VVQARLTQPA RLTSIIFAED ITTGQVLRCD AIVDLIHDIQ121IVSTTRELYL EDSPLELKIQ ALDSEGNTFS TLAGLVFEWT IVKDSEADRF SDSHNALRIL181TFLESTYIPP SYISEMEKAA KQGDTILVSG MKTGSSKLKA RIQEAVYKNV RPAEVRLLIL241ENILLNPAYD VYLMVGTSIH YKVQKIRQGK ITELSMPSDQ YELQLQNSIP GPEGDPARPV301AVLAQDTSMV TALQLGQSSL VLGHRSIRMQ GASRLPNSTI YVVEPGYLGF TVHPGDRWVL361ETGRLYEITI EVFDKFSNKV YVSDNIRIET VLPAEFFEVL SSSQNGSYHR IRALKRGQTA421IDAALTSVVD QDGGVHILQV PVWNQQEVEI HIPITLYPSI LTFPWQPKTG AYQYTIRAHG481GSGNFSWSSS SHLVATVTVK GVMTTGSDIG FSVIQAHDVQ NPLHFGEMKV YVIEPHSMEF541APCQVEARVG QALELPLRIS GLMPGGASEV VTLSDCSHFD LAVEVENQGV FQPLPGRLPP601GSEHCSGIRV KAEAQGSTTL LVSYRHGHVH LSAKITIAAY LPLKAVDPSS VALVTLGSSK661EMLFEGGPRP WILEPSKFFQ NVTAEDTDSI GLALFAPHSS RNYQQHWILV TCQALGEQVI721ALSVGNKPSL TNPFPAVEPA VVKFVCAPPS RLTLAPVYTS PQLDMSCPLL QQNKQVVPVS781SHRNPRLDLA AYDQEGRRFD NFSSLSIQWE STRPVLASIE PELPMQLVSQ DDESGQKKLH841GLQAILVHEA SGTTAITATA TGYQESHLSS ARTKQPHDPL VPLSASIELI LVEDVRVSPE901EVTIYNHPGI QAELRIREGS GYFFLNTSTA DVVKVAYQEA RGVAMVHPLL PGSSTIMIHD961LCLVFPAPAK AVVYVSDIQE LYIRVVDKVE IGKTVKAYVR VLDLHKKPFL AKYFPFMDLK1021LRAASPIITL VALDEALDNY TITFLIRGVA IGQTSLTASV TNKAGQRINS APQQIEVFPP1081FRLMPRKVTL LIGATMQVTS EGGPQPQSNI LFSISNESVA LVSAAGLVQG LAIGNGTVSG1141LVQAVDAETG KVVIISQDLV QVEVLLLRAV RIRAPIMRMR TGTQMPIYVT GITNHQNPFS1201FGNAVPGLTF HWSVTKRDVL DLRGRHHEAS IRLPSQYNFA MNVLGRVKGR TGLRVVVKAV1261DPTSGQLYGL ARELSDEIQV QVFEKLQLLN PEIEAEQILM SPNSYIKLQT NRDGAASLSY1321RVLDGPEKVP VVHVDEKGFL ASGSMIGTST IEVIAQEPFG ANQTIIVAVK VSPVSYLRVS1381MSPVLHTQNK EALVAVPLGM TVTFTVHFHD NSGDVFHAHS SVLNFATNRD DFVQIGKGPT1441NNTCVVRTVS VGLTLLRVWD AEHPGLSDFM PLPVLQAISP ELSGAMVVGD VLCLATVLTS1501LEGLSGTWSS SANSILHIDP KTGVAVARAV GSVTVYYEVA GHLRTYKEVV VSVPQRIMAR1561HLHPIQTSFQ EATASKVIVA VGDRSSNLRG ECTPTQREVI QALHPETLIS CQSQFKPAVF1621DFPSQDVFTV EPQFDTALGQ YFCSITMHRL TDKQRKHLSM KKTALVVSAS LSSSHFSTEQ1681VGAEVPFSPG LFADQAEILL SNHYTSSEIR VFGAPEVLEN LEVKSGSPAV LAFAKEKSFG1741WPSFITYTVG VLDPAAGSQG PLSTTLTFSS PVTNQAIAIP VTVAFVVDRR GPGPYGASLF1801QHFLDSYQVM FFTLFALLAG TAVMIIAYHT VCTPRDLAVP AALTPRASPG HSPHYFAASS1861PTSPNALPPA RKASPPSGLW SPAYASHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1887 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.