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NUP155 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NUP155 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NUP155 antibodies →
$504.00each
Catalog Number: LS-G102397-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NUP155 (NM_153485), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
155 kDa nucleoporin | KIAA0791 | N155 | Nucleoporin Nup155 | NUP155 | Nucleoporin 155kD | Nucleoporin 155kDa
Origin Species
Human
NCBI GenBank
NM_004298

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
155 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSSLLGAAM PASTSAAALQ EALENAGRLI DRQLQEDRMY PDLSELLMVS APNNPTVSGM
61SDMDYPLQGP GLLSVPNLPE ISSIRRVPLP PELVEQFGHM QCNCMMGVFP PISRAWLTID
121SDIFMWNYED GGDLAYFDGL SETILAVGLV KPKAGIFQPH VRHLLVLATP VDIVILGLSY
181ANLQTGSGVL NDSLSGGMQL LPDPLYSLPT DNTYLLTITS TDNGRIFLAG KDGCLYEVAY
241QAEAGWFSQR CRKINHSKSS LSFLVPSLLQ FTFSEDDPIL QIAIDNSRNI LYTRSEKGVI
301QVYDLGQDGQ GMSRVASVSQ NAIVSAAGNI ARTIDRSVFK PIVQIAVIEN SESLDCQLLA
361VTHAGVRLYF STCPFRQPLA RPNTLTLVHV RLPPGFSASS TVEKPSKVHR ALYSKGILLM
421AASENEDNDI LWCVNHDTFP FQKPMMETQM TAGVDGHSWA LSAIDELKVD KIITPLNKDH
481IPITDSPVVV QQHMLPPKKF VLLSAQGSLM FHKLRPVDQL RHLLVSNVGG DGEEIERFFK
541LHQEDQACAT CLILACSTAA CDREVSAWAT RAFFRYGGEA QMRFPTTLPP PSNVGPILGS
601PVYSSSPVPS GSPYPNPSFL GTPSHGIQPP AMSTPVCALG NPATQATNMS CVTGPEIVYS
661GKHNGICIYF SRIMGNIWDA SLVVERIFKS GNREITAIES SVPCQLLESV LQELKGLQEF
721LDRNSQFAGG PLGNPNTTAK VQQRLIGFMR PENGNPQQMQ QELQRKFHEA QLSEKISLQA
781IQQLVRKSYQ ALALWKLLCE HQFTIIVAEL QKELQEQLKI TTFKDLVIRD KELTGALIAS
841LINCYIRDNA AVDGISLHLQ DICPLLYSTD DAICSKANEL LQRSRQVQNK TEKERMLRES
901LKEYQKISNQ VDLSNVCAQY RQVRFYEGVV ELSLTAAEKK DPQGLGLHFY KHGEPEEDIV
961GLQAFQERLN SYKCITDTLQ ELVNQSKAAP QSPSVPKKPG PPVLSSDPNM LSNEEAGHHF
1021EQMLKLSQRS KDELFSIALY NWLIQVDLAD KLLQVASPFL EPHLVRMAKV DQNRVRYMDL
1081LWRYYEKNRS FSNAARVLSR LADMHSTEIS LQQRLEYIAR AILSAKSSTA ISSIAADGEF
1141LHELEEKMEV ARIQLQIQET LQRQYSHHSS VQDAVSQLDS ELMDITKLYG EFADPFKLAE
1201CKLAIIHCAG YSDPILVQTL WQDIIEKELS DSVTLSSSDR MHALSLKIVL LGKIYAGTPR
1261FFPLDFIVQF LEQQVCTLNW DVGFVIQTMN EIGVPLPRLL EVYDQLFKSR DPFWNRMKKP
1321LHLLDCIHVL LIRYVENPSQ VLNCERRRFT NLCLDAVCGY LVELQSMSSS VAVQAITGNF
1381KSLQAKLERL H
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1391 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.