LSBio
NUGGC Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NUGGC Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NUGGC antibodies →
$725.00each
Catalog Number: LS-G121730-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C8orf80(NUGGC) (NM_001010906), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAETKDVFGQ EPHPVEDDLY KERTRKRRKS DRDQRFRAFP SMEQSALKEY EKLESRTRRV
61LSNTYQKLIQ SVFLDDSIPN GVKYLINRLL ALIEKPTVDP IYIALFGSTG AGKSSLINAI
121IQQAMFLPVS GESICTSCIV QVSSGCCVQY EAKIHLLSDQ EWREELKNLT KLLHRTEELS
181REEADAWNRD EAVEEATWKL QMIYGNGAES KNYEELLRAK PKRKIPTSRV ITLKAEEAEE
241LSIKLDPYIR TQRRDWDGEA AEMRIWPLIK HVEVTLPKSD LIPEGVVLVD IPGTGDFNSK
301RDEMWKKTID KCSVIWVISD IERVSGGQAH EDLLNESIKA CQRGFCRDVA LVVTKMDKLH
361LPEYLRERKA GNQAIQSQRE AVLERNEMIK LQRTRILKEK LKRKLPADFK VLEASDLVYT
421VSAQEYWQQA LLTEEETEIP KLREYIRKSL LDKKKRTVTK YVTEAFGLLL LTDSFNSTQN
481LPNEHLHMSV LRRFAEEKVE LLEKAIAQCF ACMEQPLQEG VRTARTSYRC ILRACLVRSK
541GNQGFHQTLK AVCLKNGIYA SRTLARIDLN EALTQPVYDQ IDPVFGSIFR TGKPTGSALM
601PHIDAFKQSL QEKMTEIGIR SGWKYDSCKK NFLIQEISAI LGGLEDHILR RKRRIYESLT
661ASVQSDLKLC YEEAAQITGK KACERMKDAI RRGVDRQVAE GMFERAQERM QHQFQQLKTG
721IVEKVKGSIT TMLALASSQG DGLYKELADV GSEYKEMEKL HRSLREVAEN ARLRKGMQEF
781LLRASPSKAG PPGTSL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

796 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.