Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NTRK1 / TrkA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99267-100
Catalog Number: LS-G99267
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TrkA(NTRK1) (NM_001007792), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Oncogene TRK | TrkA/NGF receptor | Tropomyosin-related kinase A | TRK | TRK1 | Tyrosine kinase receptor | Tyrosine kinase receptor A | gp140trk | MTC | NTRK1 | p140-TrkA | Trk-A | TRKA
- NCBI GenBank
- NM_002529
- SwissProt
- P04629
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 83.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKEAALICLA PSVPPILTVK SWDTMQLRAA RSRCTNLLAA SYIENQQHLQ HLELRDLRGL61GELRNLTIVK SGLRFVAPDA FHFTPRLSRL NLSFNALESL SWKTVQGLSL QELVLSGNPL121HCSCALRWLQ RWEEEGLGGV PEQKLQCHGQ GPLAHMPNAS CGVPTLKVQV PNASVDVGDD181VLLRCQVEGR GLEQAGWILT ELEQSATVMK SGGLPSLGLT LANVTSDLNR KNVTCWAEND241VGRAEVSVQV NVSFPASVQL HTAVEMHHWC IPFSVDGQPA PSLRWLFNGS VLNETSFIFT301EFLEPAANET VRHGCLRLNQ PTHVNNGNYT LLAANPFGQA SASIMAAFMD NPFEFNPEDP361IPDTNSTSGD PVEKKDETPF GVSVAVGLAV FACLFLSTLL LVLNKCGRRN KFGINRPAVL421APEDGLAMSL HFMTLGGSSL SPTEGKGSGL QGHIIENPQY FSDACVHHIK RRDIVLKWEL481GEGAFGKVFL AECHNLLPEQ DKMLVAVKAL KEASESARQD FQREAELLTM LQHQHIVRFF541GVCTEGRPLL MVFEYMRHGD LNRFLRSHGP DAKLLAGGED VAPGPLGLGQ LLAVASQVAA601GMVYLAGLHF VHRDLATRNC LVGQGLVVKI GDFGMSRDIY STDYYRVGGR TMLPIRWMPP661ESILYRKFTT ESDVWSFGVV LWEIFTYGKQ PWYQLSNTEA IDCITQGREL ERPRACPPEV721YAIMRGCWQR EPQQRHSIKD VHARLQALAQ APPVYLDVLGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
760 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.