Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NTNG1 / Netrin G1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G124267-100
Catalog Number: LS-G124267
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Netrin G1(NTNG1) (NM_001113226), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Axon guidance molecule | Laminet 1 | Laminet-1 | Lmnt1 | Netrin G1 | Netrin G1f | NTNG1 | KIAA0976 | YLSR571 | Netrin-G1
- Origin Species
- Human
- NCBI GenBank
- AB023193
- SwissProt
- Q9Y2I2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MYLSRFLSIH ALWVTVSSVM QPYPLVWGHY DLCKTQIYTE EGKVWDYMAC QPESTDMTKY61LKVKLDPPDI TCGDPPETFC AMGNPYMCNN ECDASTPELA HPPELMFDFE GRHPSTFWQS121ATWKEYPKPL QVNITLSWSK TIELTDNIVI TFESGRPDQM ILEKSLDYGR TWQPYQYYAT181DCLDAFHMDP KSVKDLSQHT VLEIICTEEY STGYTTNSKI IHFEIKDRFA FFAGPRLRNM241ASLYGQLDTT KKLRDFFTVT DLRIRLLRPA VGEIFVDELH LARYFYAISD IKVRGRCKCN301LHATVCVYDN SKLTCECEHN TTGPDCGKCK KNYQGRPWSP GSYLPIPKGT ANTCIPSISS361IGNCECFGHS NRCSYIDLLN TVICVSCKHN TRGQHCELCR LGYFRNASAQ LDDENVCIEC421YCNPLGSIHD RCNGSGFCEC KTGTTGPKCD ECLPGNSWHY GCQPNVCDNE LLHCQNGGTC481HNNVRCLCPA AYTGILCEKL RCEEAGSCGS DSGQGAPPHG SPALLLLTTL LGTASPLVFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
539 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.