Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NTN3 / NTN2L Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115482-100
Catalog Number: LS-G115482
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NTN3 (NM_006181), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Netrin-3 | Netrin 2 (chicken)-like | Netrin 2-like (chicken) | Netrin-2-like protein | NTN2L | NTN3 | Netrin 3
- Origin Species
- Human
- NCBI GenBank
- NM_006181
- SwissProt
- O00634
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPGWPWGLLL TAGTLFAALS PGPPAPADPC HDEGGAPRGC VPGLVNAALG REVLASSTCG61RPATRACDAS DPRRAHSPAL LTSPGGTASP LCWRSESLPR APLNVTLTVP LGKAFELVFV121SLRFCSAPPA SVALLKSQDH GRSWAPLGFF SSHCDLDYGR LPAPANGPAG PGPEALCFPA181PLAQPDGSGL LAFSMQDSSP PGLDLDSSPV LQDWVTATDV RVVLTRPSTA GDPRDMEAVV241PYSYAATDLQ VGGRCKCNGH ASRCLLDTQG HLICDCRHGT EGPDCGRCKP FYCDRPWQRA301TARESHACLA CSCNGHARRC RFNMELYRLS GRRSGGVCLN CRHNTAGRHC HYCREGFYRD361PGRALSDRRA CRACDCHPVG AAGKTCNQTT GQCPCKDGVT GLTCNRCAPG FQQSRSPVAP421CVKTPIPGPT EDSSPVQPQD CDSHCKPARG SYRISLKKFC KKDYAVQVAV GARGEARGAW481TRFPVAVLAV FRSGEERARR GSSALWVPAG DAACGCPRLL PGRRYLLLGG GPGAAAGGAG541GRGPGLIAAR GSLVLPWRDA WTRRLRRLQR RERRGRCSAASGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
580 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.