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NT5C1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NT5C1B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NT5C1B antibodies →
$725.00each
Catalog Number: LS-G108356-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NT5C1B (NM_033253), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
5-nucleotidase | cytosolic IB | 5'-nucleotidase | AIRP | CN1B | Cytosolic 5-nucleotidase 1B | Cytosolic 5-nucleotidase IB | CN-IB | NT5C1B
Origin Species
Human
NCBI GenBank
NM_001002006
SwissProt
Q96P26

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSQTSLKQKK NEPGMRSSKE SLEAEKRKES DKTGVRLSNQ GSQESSLRKT DSRGYLVRSQ
61WSRISRSPST KAPSIDEPRS RNTSAKLPSS STSSRTPSTS PSLHDSSPPP LSGQPSLQPP
121ASPQLPRSLD SRPPTPPEPD PGSRRSTKMQ ENPEAWAQGI VREIRQTRDS QPLEYSRTSP
181TEWKSSSQRR GIYPASTQLD RNSLSEQQQQ QREDEDDYEA AYWASMRSFY EKNPSCSRPW
241PPKPKNAITI ALSSCALFNM VDGRKIYEQE GLEKYMEYQL TNENVILTPG PAFRFVKALQ
301YVNARLRDLY PDEQDLFDIV LMTNNHAQVG VRLINSVNHY GLLIDRFCLT GGKDPIGYLK
361AYLTNLYIAA DSEKVQEAIQ EGIASATMFD GAKDMAYCDT QLRVAFDGDA VLFSDESEHF
421TKEHGLDKFF QYDTLCESKP LAQGPLKGFL EDLGRLQKKF YAKNERLLCP IRTYLVTARS
481AASSGARVLK TLRRWGLEID EALFLAGAPK SPILVKIRPH IFFDDHMFHI EGAQRLGSIA
541AYGFNKKFSS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

550 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.