Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NSMF / NELF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113156-100
Catalog Number: LS-G113156
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NELF(NSMF) (NM_015537), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HH9 | Nasal embryonic LHRH factor | NELF | RP11-48C7.1
- Origin Species
- Human
- NCBI GenBank
- NM_015537
- SwissProt
- Q6X4W1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGAAASRRRA LRSEAMSSVA AKVRAARAFG EYLSQSHPEN RNGADHLLAD AYSGHDGSPE61MQPAPQNKRR LSLVSNGCYE GSLSEEPSIR KPAGEGPQPR VYTISGEPAL LPSPEAEAIE121LAVVKGRRQR HPHHHSQPLR ASPGGSREDV SRPCQSWAGS RQGSKECPGC AQLAPGPTPR181AFGLDQPPLP ETSGRRKKLE RMYSVDRVSD DIPIRTWFPK ENLFSFQTAT TTMQAVFRGY241AERKRRKREN DSASVIQRNF RKHLRMVGSR RVKAQTFAER RERSFSRSWS DPTPMKADTS301HDSRDSSDLQ SSHCTLDEAF EDLDWDTEKG LEAVACDTEG FVPPKVMLIS SKVPKAEYIP361TIIRRDDPSI IPILYDHEHA TFEDILEEIE RKLNVYHKGA KIWKMLIFCQ GGPGHLYLLK421NKVATFAKVE KEEDMIHFWK RLSRLMSKVN PEPNVIHIMG CYILGNPNGE KLFQNLRTLM481TPYRVTFESP LELSAQGKQM IETYFDFRLY RLWKSRQHSK LLDFDDVLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
528 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.