Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NSMCE4A / NSE4A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112350-100
Catalog Number: LS-G112350
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NSMCE4A (NM_017615), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C10orf86 | Non-SMC element 4 homolog A | NS4EA | BA500G22.3 | NSE4A | NSMCE4A
- Origin Species
- Human
- NCBI GenBank
- NM_017615
- SwissProt
- Q9NXX6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGDSSGRGP EGRGRGRDPH RDRTRSRSRS RSPLSPRSRR GSARERREAP ERPSLEDTEP61SDSGDEMMDP ASLEAEADQG LCRQIRHQYR ALINSVQQNR EDILNAGDKL TEVLEEANTL121FNEVSRAREA VLDAHFLVLA SDLGKEKAKQ LRSDLSSFDM LRYVETLLTH MGVNPLEAEE181LIRDEDSPDF EFIVYDSWKI TGRTAENTFN KTHTFHFLLG SIYGECPVPK PRVDRPRKVP241VIQEERAMPA QLRRMEESHQ EATEKEVERI LGLLQTYFRE DPDTPMSFFD FVVDPHSFPR301TVENIFHVSF IIRDGFARIR LDQDRLPVIE PVSINEENEG FEHNTQVRNQ GIIALSYRDW361EIVKTFEISE PVITPSQRQQ KPSATRTRPLEQKLISEEDLAANDILDYKDDDDKV
384 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.