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NRXN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NRXN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NRXN2 antibodies →
$725.00each
Catalog Number: LS-G113486-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Neurexin II alpha(NRXN2) (NM_015080), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0921 | Neurexin II-alpha | Neurexin-2-beta | Neurexin II | Neurexin II-beta | NRXN2 | Neurexin 2 | Neurexin-2-alpha
Origin Species
Human
NCBI GenBank
NM_015080
SwissProt
Q9P2S2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
184.98 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASGSRWRPT PPPLLLLLLL ALAARADGLE FGGGPGQWAR YARWAGAASS GELSFSLRTN
61ATRALLLYLD DGGDCDFLEL LLVDGRLRLR FTLSCAEPAT LQLDTPVADD RWHMVLLTRD
121ARRTALAVDG EARAAEVRSK RREMQVASDL FVGGIPPDVR LSALTLSTVK YEPPFRGLLA
181NLKLGERPPA LLGSQGLRGA TADPLCAPAR NPCANGGLCT VLAPGEVGCD CSHTGFGGKF
241CSEEEHPMEG PAHLTLNSEV GSLLFSEGGA GRGGAGDVHQ PTKGKEEFVA TFKGNEFFCY
301DLSHNPIQSS TDEITLAFRT LQRNGLMLHT GKSADYVNLS LKSGAVWLVI NLGSGAFEAL
361VEPVNGKFND NAWHDVRVTR NLRQHAGIGH AMVNKLHYLV TISVDGILTT TGYTQEDYTM
421LGSDDFFYIG GSPNTADLPG SPVSNNFMGC LKDVVYKNND FKLELSRLAK EGDPKMKLQG
481DLSFRCEDVA ALDPVTFESP EAFVALPRWS AKRTGSISLD FRTTEPNGLL LFSQGRRAGG
541GAGSHSSAQR ADYFAMELLD GHLYLLLDMG SGGIKLRASS RKVNDGEWCH VDFQRDGRKG
601SISVNSRSTP FLATGDSEIL DLESELYLGG LPEGGRVDLP LPPEVWTAAL RAGYVGCVRD
661LFIDGRSRDL RGLAEAQGAV GVAPFCSRET LKQCASAPCR NGGVCREGWN RFICDCIGTG
721FLGRVCEREA TVLSYDGSMY MKIMLPNAMH TEAEDVSLRF MSQRAYGLMM ATTSRESADT
781LRLELDGGQM KLTVNLDCLR VGCAPSKGPE TLFAGHKLND NEWHTVRVVR RGKSLQLSVD
841NVTVEGQMAG AHMRLEFHNI ETGIMTERRF ISVVPSNFIG HLSGLVFNGQ PYMDQCKDGD
901ITYCELNARF GLRAIVADPV TFKSRSSYLA LATLQAYASM HLFFQFKTTA PDGLLLFNSG
961NGNDFIVIEL VKGYIHYVFD LGNGPSLMKG NSDKPVNDNQ WHNVVVSRDP GNVHTLKIDS
1021RTVTQHSNGA RNLDLKGELY IGGLSKNMFS NLPKLVASRD GFQGCLASVD LNGRLPDLIA
1081DALHRIGQVE RGCDGPSTTC TEESCANQGV CLQQWDGFTC DCTMTSYGGP VCNDPGTTYI
1141FGKGGALITY TWPPNDRPST RMDRLAVGFS THQRSAVLVR VDSASGLGDY LQLHIDQGTV
1201GVIFNVGTDD ITIDEPNAIV SDGKYHVVRF TRSGGNATLQ VDSWPVNERY PAGNFDNERL
1261AIARQRIPYR LGRVVDEWLL DKGRQLTIFN SQAAIKIGGR DQGRPFQGQV SGLYYNGLKV
1321LALAAESDPN VRTEGHLRLV GEGPSVLLSA ETTATTLLAD MATTIMETTT TMATTTTRRG
1381RSPTLRDSTT QNTDDLLVAS AECPSDDEDL EECEPSTGGE LILPIITEDS LDPPPVATRS
1441PFVPPPPTFY PFLTGVGATQ DTLPPPAARR PPSGGPCQAE RDDSDCEEPI EASGFASGEV
1501FDSSLPPTDD EDFYTTFPLV TDRTTLLSPR KPAPRPNLRT DGATGAPGVL FAPSAPAPNL
1561PAGKMNHRDP LQPLLENPPL GPGAPTSFEP RRPPPLRPGV TSAPGFPHLP TANPTGPGER
1621GPPGAVEVIR ESSSTTGMVV GIVAAAALCI LILLYAMYKY RNRDEGSYQV DQSRNYISNS
1681AQSNGAVVKE KAPAAPKTPS KAKKNKDKEY YV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1712 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.