Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NRP2 / Neuropilin 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102590-100
Catalog Number: LS-G102590
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NRP2 (NM_201266), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Neuropilin-2a(22) | NPN2 | NP2 | NRP2 | Neuropilin 2 | Neuropilin-2 | Neuropilin-2b(0) | VEGF165R2 | VEGF1265R2 | Neuropilin-2a(17) | PRO2714
- Origin Species
- Human
- NCBI GenBank
- NM_201266
- SwissProt
- O60462
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 102.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTFYSPAVMN YSVPGSTSNL DGGPVRLSTS PNVLWPTSGH LSPLATHCQS SLLYAEPQKS61PWCEARSLEH TLPVNRETLK RKLSGSSCAS PVTSPNAKRD AHFCPVCSDY ASGYHYGVWS121CEGCKAFFKR SIQGHNDYIC PATNQCTIDK NRRKSCQACR LRKCYEVGMV KCGSRRERCG181YRIVRRQRSS SEQVHCLSKA KRNGGHAPRV KELLLSTLSP EQLVLTLLEA EPPNVLVSRP241SMPFTEASMM MSLTKLADKE LVHMIGWAKK IPGFVELSLL DQVRLLESCW MEVLMVGLMW301RSIDHPGKLI FAPDLVLDRD EGKCVEGILE IFDMLLATTS RFRELKLQHK EYLCVKAMIL361LNSSMYPLAS ANQEAESSRK LTHLLNAVTD ALVWVIAKSG ISSQQQSVRL ANLLMLLSHV421RHISNKGMEH LLSMKCKNVV PVYDLLLEML NAHTLRGYKS SISGSECSST EDSKNKESSQ481NLQSQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
485 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.