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NRG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NRG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G128190-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NRG3 (NM_001165973), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Neuregulin-3-like polypeptide | Neuregulin 3 | NRG3 | Pro-NRG3 | HRG3
Origin Species
Human
NCBI GenBank
NM_001010848
SwissProt
P56975

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MECGIPPTLV CVGRGGGLHT INIIIWYYFP SAWRTCFNIS SSVGLLLTNS YKFYTTTYST
61ERSEHFKPCR DKDLAYCLND GECFVIETLT GSHKHCRCKE GYQGVRCDQF LPKTDSILSD
121PTDHLGIEFM ESEEVYQRQV LSISCIIFGI VIVGMFCAAF YFKSKKQAKQ IQEQLKVPQN
181GKSYSLKASS TMAKSENLVK SHVQLQNYSK VERHPVTALE KMMESSFVGP QSFPEVPSPD
241RGSQSVKHHR SLSSCCSPGQ RSGMLHRNAF RRTPPSPRSR LGGIVGPAYQ QLEESRIPDQ
301DTIPCQGIEV RKTISHLPIQ LWCVERPLDL KYSSSGLKTQ RNTSINMQLP SRETNPYFNS
361LEQKDLVGYS STRASSVPII PSVGLEETCL QMPGISEVKS IKWCKNSYSA DVVNVSIPVS
421DCLIAEQQEV KILLETVQEQ IRILTDARRS EDYELASVET EDSASENTAF LPLSPTAKSE
481REAQFVLRNE IQRDSALTK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

499 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.