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NRG2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NRG2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G114233-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NRG2 (NM_013982), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DON1 | NRG2 | HRG2 | Neuregulin 2 | Pro-NRG2 | Divergent of neuregulin-1 | Don-1 | NTAK
Origin Species
Human
NCBI GenBank
NM_013982
SwissProt
O14511

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRQVCCSALP PPPLEKGRCS SYSDSSSSSS ERSSSSSSSS SESGSSSRSS SNNSSISRPA
61APPEPRPQQQ PQPRSPAARR AAARSRAAAA GGMRRDPAPG FSMLLFGVSL ACYSPSLKSV
121QDQAYKAPVV VEGKVQGLVP AGGSSSNSTR EPPASGRVAL VKVLDKWPLR SGGLQREQVI
181SVGSCVPLER NQRYIFFLEP TEQPLVFKTA FAPLDTNGKN LKKEVGKILC TDCATRPKLK
241KMKSQTGQVG EKQSLKCEAA AGNPQPSYRW FKDGKELNRS RDIRIKYGNG RKNSRLQFNK
301VKVEDAGEYV CEAENILGKD TVRGRLYVNS VSTTLSSWSG HARKCNETAK SYCVNGGVCY
361YIEGINQLSC KCPNGFFGQR CLEKLPLRLY MPDPKQKHLG FELKEAEELY QKRVLTITGI
421CVALLVVGIV CVVAYCKTKK QRKQMHNHLR QNMCPAHQNR SLANGPSHPR LDPEEIQMAD
481YISKNVPATD HVIRRETETT FSGSHSCSPS HHCSTATPTS SHRHESHTWS LERSESLTSD
541SQSGIMLSSV GTSKCNSPAC VEARARRAAA YNLEERRRAT APPYHDSVDS LRDSPHSERY
601VSALTTPARL SPVDFHYSLA TQVPTFEITS PNSAHAVSLP PAAPISYRLA EQQPLLRHPA
661PPGPGPGPGP GPGPGADMQR SYDSYYYPAA GPGPRRGTCA LGGSLGSLPA SPFRIPEDDE
721YETTQECAPP PPPRPRARGA SRRTSAGPRR WRRSRLNGLA AQRARAARDS LSLSSGSGGG
781SASASDDDAD DADGALAAES TPFLGLRGAH DALRSDSPPL CPAADSRTYY SLDSHSTRAS
841SRHSRGPPPR AKQDSAPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

858 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.