Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NRG1 / Heregulin / Neuregulin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114218-100
Catalog Number: LS-G114218
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NRG1 (NM_013956), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARIA | GGF | GGF2 | Glial growth factor | Heregulin | HRGA | MSTP131 | NDF | Neu differentiation factor | Neuregulin 1 type IV beta 3 | Neuregulin 1 type IV beta 1a | HRG1 | NRG-1 | NRG1 | SMDF | Neuregulin 1 | Pro-NRG1 | HGL | MST131
- Origin Species
- Human
- NCBI GenBank
- NM_013964
- SwissProt
- Q02297
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSERKEGRGK GKGKKKERGS GKKPESAAGS QSPALPPQLK EMKSQESAAG SKLVLRCETS61SEYSSLRFKW FKNGNELNRK NKPQNIKIQK KPGKSELRIN KASLADSGEY MCKVISKLGN121DSASANITIV ESNEIITGMP ASTEGAYVSS ESPIRISVST EGANTSSSTS TSTTGTSHLV181KCAEKEKTFC VNGGECFMVK DLSNPSRYLC KCPNEFTGDR CQNYVMASFY KHLGIEFMEA241EELYQKRVLT ITGICIALLV VGIMCVVAYC KTKKQRKKLH DRLRQSLRSE RNNMMNIANG301PHHPNPPPEN VQLVNQYVSK NVISSEHIVE REAETSFSTS HYTSTAHHST TVTQTPSHSW361SNGHTESILS ESHSVIVMSS VENSRHSSPT GGPRGRLNGT GGPRECNSFL RHARETPDSY421RDSPHSERYV SAMTTPARMS PVDFHTPSSP KSPPSEMSPP VSSMTVSMPS MAVSPFMEEE481RPLLLVTPPR LREKKFDHHP QQFSSFHHNP AHDSNSLPAS PLRIVEDEEY ETTQEYEPAQ541EPVKKLANSR RAKRTKPNGH IANRLEVDSN TSSQSSNSES ETEDERVGED TPFLGIQNPL601AASLEATPAF RLADSRTNPA VRFSTQEEIQ ARLSSVIANQ DPIAVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
645 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.