Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NRD1 / Nardilysin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117924-100
Catalog Number: LS-G117924
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Nardilysin(NRDC) (NM_002525), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- N-arginine dibasic convertase | Nardilysin | HNRD1 | HNRD2 | NRD convertase | NRDC | NRD1 | NRD-C
- Origin Species
- Human
- NCBI GenBank
- NM_002525
- SwissProt
- O43847
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 139.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLRRVTVAAV CATRRKLCEA GRDVAALWGI ETRGRCEDSA AARPFPILAM PGRNKAKSTC61SCPDLQPNGQ DLGENSRVAR LGADESEEEG RRGSLSNAGD PEIVKSPSDP KQYRYIKLQN121GLQALLISDL SNMEGKTGNT TDDEEEEEVE EEEEDDDEDS GAEIEDDDEE GFDDEDEFDD181EHDDDLDTED NELEELEERA EARKKTTEKQ QLQSLFLLWS KLTDRLWFKS TYSKMSSTLL241VETRNLYGVV GAESRSAPVQ HLAGWQAEEQ QGETDTVLSA AALCVGVGSF ADPDDLPGLA301HFLEHMVFMG SLKYPDENGF DAFLKKHGGS DNASTDCERT VFQFDVQRKY FKEALDRWAQ361FFIHPLMIRD AIDREVEAVD SEYQLARPSD ANRKEMLFGS LARPGHPMGK FFWGNAETLK421HEPRKNNIDT HARLREFWMR YYSSHYMTLV VQSKETLDTL EKWVTEIFSQ IPNNGLPRPN481FGHLTDPFDT PAFNKLYRVV PIRKIHALTI TWALPPQQQH YRVKPLHYIS WLVGHEGKGS541ILSFLRKKCW ALALFGGNGE TGFEQNSTYS VFSISITLTD EGYEHFYEVA YTVFLYLKML601QKLGPEKRIF EEIRKIEDNE FHYQEQTDPV EYVENMCENM QLYPLQDILT GDQLLFEYKP661EVIGEALNQL VPQKANLVLL SGANEGKCDL KEKWFGTQYS IEDIENSWAE LWNSNFELNP721DLHLPAENKY IATDFTLKAF DCPETEYPVK IVNTPQGCLW YKKDNKFKIP KAYIRFHLIS781PLIQKSAANV VLFDIFVNIL THNLAEPAYE ADVAQLEYKL AAGEHGLIIR VKGFNHKLPL841LFQLIIDYLA EFNSTPAVFT MITEQLKKTY FNILIKPETL AKDVRLLILE YARWSMIDKY901QALMDGLSLE SLLSFVKEFK SQLFVEGLVQ GNVTSTESMD FLKYVVDKLN FKPLEQEMPV961QFQVVELPSG HHLCKVKALN KGDANSEVTV YYQSGTRSLR EYTLMELLVM HMEEPCFDFL1021RTKQTLGYHV YPTCRNTSGI LGFSVTVGTQ ATKYNSEVVD KKIEEFLSSF EEKIENLTEE1081AFNTQVTALI KLKECEDTHL GEEVDRNWNE VVTQQYLFDR LAHEIEALKS FSKSDLVNWF1141KAHRGPGSKM LSVHVVGYGK YELEEDGSPS SEDSNSSCEV MQLTYLPTSP LLADCIIPIT1201DIRAFTTTLN LLPYHKIVKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1219 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.