Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NR6A1 / GCNF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118551-100
Catalog Number: LS-G118551
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GCNF(NR6A1) (NM_001489), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Germ cell nuclear factor | HGCNF | NR61 | HRTR | RTR | Ncnf | GCNF | GCNF1 | NR6A1
- Origin Species
- Human
- NCBI GenBank
- NM_033334
- SwissProt
- Q15406
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MERDEPPPSG GGGGGGSAGF LEPPAALPPP PRNGFCQDEL AELDPGTNDR AEQRTCLICG61DRATGLHYGI ISCEGCKGFF KRSICNKRVY RCSRDKNCVM SRKQRNRCQY CRLLKCLQMG121MNRKAIREDG MPGGRNKSIG PVQISEEEIE RIMSGQEFEE EANHWSNHGD SDHSSPGNRA181SESNQPSPGS TLSSRSVELN GFMAFREQYM GMSVPPHYQY IPHLFSYSGH SPLLPQQARS241LDPQSYSLIH QLLSAEDLEP LGTPMLIEDG YAVTQAELFA LLCRLADELL FRQIAWIKKL301PFFCELSIKD YTCLLSSTWQ ELILLSSLTV YSKQIFGELA DVTAKYSPSD EELHRFSDEG361MEVIERLIYL YHKFHQLKVS NEEYACMKAI NFLNQDIRGL TSASQLEQLN KRYWYICQDF421TEYKYTHQPN RFPDLMMCLP EIRYIAGKMV NVPLEQLPLL FKVVLHSCKT SVGKETRTRPLEQKLISEEDLAANDILDYKDDDDKV
475 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.