Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NR1H4 / FXR Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G100461-100
Catalog Number: LS-G100461
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Bile Acid Receptor(NR1H4) (NM_005123), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BAR | Farnesyl x receptor | FXR | HRR1 | Farnesoid x activated receptor | Farnesoid X receptor | Farnesol receptor HRR-1 | HRR-1 | RXR-interacting protein 14 | NR1H4 | RIP14 | Bile acid receptor | Farnesoid X nuclear receptor | Farnesoid X-activated receptor
- Origin Species
- Human
- NCBI GenBank
- NM_005123
- SwissProt
- Q96RI1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSKMNLIEH SHLPTTDEFS FSENLFGVLT EQVAGPLGQN LEVEPYSQYS NVQFPQVQPQ61ISSSSYYSNL GFYPQQPEEW YSPGIYELRR MPAETLYQGE TEVAEMPVTK KPRMGASAGR121IKGDELCVVC GDRASGYHYN ALTCEGCKGF FRRSITKNAV YKCKNGGNCV MDMYMRRKCQ181ECRLRKCKEM GMLAECLLTE IQCKSKRLRK NVKQHADQTV NEDSEGRDLR QVTSTTKSCR241EKTELTPDQQ TLLHFIMDSY NKQRMPQEIT NKILKEEFSA EENFLILTEM ATNHVQVLVE301FTKKLPGFQT LDHEDQIALL KGSAVEAMFL RSAEIFNKKL PSGHSDLLEE RIRNSGISDE361YITPMFSFYK SIGELKMTQE EYALLTAIVI LSPDRQYIKD REAVEKLQEP LLDVLQKLCK421IHQPENPQHF ACLLGRLTEL RTFNHHHAEM LMSWRVNDHK FTPLLCEIWD VQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
472 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.