Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NR1H2 / LXR Beta Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100977-100
Catalog Number: LS-G100977
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LXR beta(NR1H2) (NM_007121), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Liver X receptor beta | LXR beta | LXR-b | LXRB | Lxrbeta | NER | Nuclear receptor NER | LX receptor beta | NR1H2 | Ubiquitous receptor | RIP15 | Liver X nuclear receptor beta | NER-I | Oxysterols receptor LXR-beta | UNR
- Origin Species
- Human
- NCBI GenBank
- NM_007121
- SwissProt
- P55055
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSPTTSSLD TPLPGNGPPQ PGAPSSSPTV KEEGPEPWPG GPDPDVPGTD EASSACSTDW61VIPDPEEEPE RKRKKGPAPK MLGHELCRVC GDKASGFHYN VLSCEGCKGF FRRSVVRGGA121RRYACRGGGT CQMDAFMRRK CQQCRLRKCK EAGMREQCVL SEEQIRKKKI RKQQQQESQS181QSQSPVGPQG SSSSASGPGA SPGGSEAGSQ GSGEGEGVQL TAAQELMIQQ LVAAQLQCNK241RSFSDQPKVT PWPLGADPQS RDARQQRFAH FTELAIISVQ EIVDFAKQVP GFLQLGREDQ301IALLKASTIE IMLLETARRY NHETECITFL KDFTYSKDDF HRAGLQVEFI NPIFEFSRAM361RRLGLDDAEY ALLIAINIFS ADRPNVQEPG RVEALQQPYV EALLSYTRIK RPQDQLRFPR421MLMKLVSLRT LSSVHSEQVF ALRLQDKKLP PLLSEIWDVH ETRTRPLEQKLISEEDLAANDILDYKDDDDKV
461 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.