LSBio
NR1D2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NR1D2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NR1D2 antibodies →
$504.00each
Catalog Number: LS-G116148-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NR1D2 (NM_005126), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EAR-1R | HZF2 | NR1D2 | Nuclear receptor rvr | Rev-erbabeta | Rev-erb-beta | Rev-erba beta | RVR | BD73 | Nuclear receptor ear-1r
Origin Species
Human
NCBI GenBank
D16815
SwissProt
Q14995

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEVNAGGVIA YISSSSSASS HASCHSEGSE NSFQSSSSSV PSSPNSSNSD TNGNPKNGDL
61ANIEGILKND RIDCSMKTSK SSAPGMTKSH SGVTKFSGMV LLCKVCGDVA SGFHYGVHAC
121EGCKGFFRRS IQQNIQYKKC LKNENCSIMR MNRNRCQQCR FKKCLSVGMS RDAVRFGRIP
181KREKQRMLIE MQSAMKTMMN SQFSGHLQND TLVEHHEQTA LPAQEQLRPK PQLEQENIKS
241SSPPSSDFAK EEVIGMVTRA HKDTFMYNQE QQENSAESMQ PKRGERIRKN MEQYNLNHDH
301CGNGLSSHFP CSESQQHLNG QFKGRNIMHY PNGHAICIAN GHCMNFSNAY TQRVCDRVPI
361DGFSQNENKN SYLCNTGGRM HLVCPMSKSP YVDPHKSGHE IWEEFSMSFT PAVKEVVEFA
421KRIPGFRDLS QHDQVNLLKA GTFEVLMVRF ASLFDAKERT VTFLSGKKYS VDDLHSMGAG
481DLLNSMFEFS EKLNALQLSD EEMSLFTAVV LVSADRSGIE NVNSVEALQE TLIRALRTLI
541MKNHPNEASI FTKLLLKLPD LRSLNNMHSE ELLAFKVHP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

579 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.