Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NPSR1 / NPSR / GPR154 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102844-100
Catalog Number: LS-G102844
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NPSR1 (NM_207172), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ASRT2 | G protein-coupled receptor 154 | G-protein coupled receptor 154 | GPR154 | Neuropeptide S receptor 1 | NPSR | NPSR1 | PGR14 | GPRA | Neuropeptide S receptor | VRR1
- Origin Species
- Human
- NCBI GenBank
- NM_207172
- SwissProt
- Q6W5P4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPANFTEGSF DSSGTGQTLD SSPVACTETV TFTEVVEGKE WGSFYYSFKT EQLITLWVLF61VFTIVGNSVV LFSTWRRKKK SRMTFFVTQL AITDSFTGLV NILTDINWRF TGDFTAPDLV121CRVVRYLQVV LLYASTYVLV SLSIDRYHAI VYPMKFLQGE KQARVLIVIA WSLSFLFSIP181TLIIFGKRTL SNGEVQCWAL WPDDSYWTPY MTIVAFLVYF IPLTIISIMY GIVIRTIWIK241SKTYETVISN CSDGKLCSSY NRGLISKAKI KAIKYSIIII LAFICCWSPY FLFDILDNFN301LLPDTQERFY ASVIIQNLPA LNSAINPLIY CVFSSSISFP CREQRSQDSR MTFRERTERH361EMQILSKPEF ITRTRPLEQKLISEEDLAANDILDYKDDDDKV
371 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.