Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NPR2L / TUSC4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115240-100
Catalog Number: LS-G115240
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NPR2L(NPRL2) (NM_006545), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G21 protein | Gene 21 protein | NPR2-like protein | NPR2L | TUSC4 | NPRL2 | Tumor suppressor candidate 4
- Origin Species
- Human
- NCBI GenBank
- NM_006545
- SwissProt
- Q8WTW4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSGCRIECI FFSEFHLTLG PKITYQVPED FISRELFDTV QVYIITKPEL QNKLITVTAM61EKKLIGCPVC IEHKKYSRNA LLFNLGFVCD AQAKTCALEP IVKKLAGYLT TLELESSFVS121MEESKQKLVP IMTILLEELN ASGRCTLPID ESNTIHLKVI EQRPDPPVAQ EYDVPVFTKD181KEDFFNSQWD LTTQQILPYI DGFRHIQKIS AEADVELNLV RIAIQNLLYY GVVTLVSILQ241YSNVYCPTPK VQDLVDDKSL QEACLSYVTK QGHKRASLRD VFQLYCSLSP GTTVRDLIGR301HPQQLQHVDE RKLIQFGLMK NLIRRLQKYP VRVTREEQSH PARLYTGCHS YDEICCKTGM361SYHELDERLE NDPNIIICWKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
380 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.