Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NPFFR1 / GPR147 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110438-100
Catalog Number: LS-G110438
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NPFF1 Receptor(NPFFR1) (NM_022146), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G-protein coupled receptor 147 | GPR147 | Neuropeptide FF 1 | Neuropeptide FF receptor 1 | NPFF-R1 | NPFFR1 | OT7T022 | NPFF1 receptor | G protein-coupled receptor 147 | Neuropeptide FF1 receptor | NPFF1 | NPFF1R1
- Origin Species
- Human
- NCBI GenBank
- NM_022146
- SwissProt
- Q9GZQ6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGEPSQPPN SSWPLSQNGT NTEATPATNL TFSSYYQHTS PVAAMFIVAY ALIFLLCMVG61NTLVCFIVLK NRHMHTVTNM FILNLAVSDL LVGIFCMPTT LVDNLITGWP FDNATCKMSG121LVQGMSVSAS VFTLVAIAVE RFRCIVHPFR EKLTLRKALV TIAVIWALAL LIMCPSAVTL181TVTREEHHFM VDARNRSYPL YSCWEAWPEK GMRRVYTTVL FSHIYLAPLA LIVVMYARIA241RKLCQAPGPA PGGEEAADPR ASRRRARVVH MLVMVALFFT LSWLPLWALL LLIDYGQLSA301PQLHLVTVYA FPFAHWLAFF NSSANPIIYG YFNENFRRGF QAAFRARLCP RPSGSHKEAY361SERPGGLLHR RVFVVVRPSD SGLPSESGPS SGAPRPGRLP LRNGRVAHHG LPREGPGCSH421LPLTIPAWDITRTRPLEQKLISEEDLAANDILDYKDDDDKV
430 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.