Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NPBWR1 / GPR7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100511-100
Catalog Number: LS-G100511
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR7(NPBWR1) (NM_005285), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 7 | GPR7 | Neuropeptide B/W receptor 1 | Neuropeptides B/W receptor 1 | NPBWR1 | G-protein coupled receptor 7
- Origin Species
- Human
- NCBI GenBank
- NM_005285
- SwissProt
- P48145
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDNASFSEPW PANASGPDPA LSCSNASTLA PLPAPLAVAV PVVYAVICAV GLAGNSAVLY61VLLRAPRMKT VTNLFILNLA IADELFTLVL PINIADFLLR QWPFGELMCK LIVAIDQYNT121FSSLYFLTVM SADRYLVVLA TAESRRVAGR TYSAARAVSL AVWGIVTLVV LPFAVFARLD181DEQGRRQCVL VFPQPEAFWW RASRLYTLVL GFAIPVSTIC VLYTTLLCRL HAMRLDSHAK241ALERAKKRVT FLVVAILAVC LLCWTPYHLS TVVALTTDLP QTPLVIAISY FITSLSYANS301CLNPFLYAFL DASFRRNLRQ LITCRAAASGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
328 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.