LSBio
NOX5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NOX5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NOX5 antibodies →
$725.00each
Catalog Number: LS-G101881-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NOX5 (NM_024505), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NADPH oxidase 5 | NOX5 | NOX5A | NOX5B
Origin Species
Human
NCBI GenBank
NM_024505
SwissProt
Q96PH1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAEEDARWL RWVTQQFKTI AGEDGEISLQ EFKAALHVKE SFFAERFFAL FDSDRSGTIT
61LQELQEALTL LIHGSPMDKL KFLFQVYDID VCARQGASAG TEWGAGAGPH WASSPLGTGS
121GSIDPDELRT VLQSCLRESA ISLPDEKLDQ LTLALFESAD ADGNGAITFE ELRDELQRFP
181GVMENLTISA AHWLTAPAPR PRPRRPRQLT RAYWHNHRSQ LFCLATYAGL HVLLFGLAAS
241AHRDLGASVM VAKGCGQCLN FDCSFIAVLM LRRCLTWLRA TWLAQVLPLD QNIQFHQLMG
301YVVVGLSLVH TVAHTVNFVL QAQAEASPFQ FWELLLTTRP GIGWVHGSAS PTGVALLLLL
361LLMFICSSSC IRRSGHFEVF YWTHLSYLLV WLLLIFHGPN FWKWLLVPGI LFFLEKAIGL
421AVSRMAAVCI MEVNLLPSKV THLLIKRPPF FHYRPGDYLY LNIPTIARYE WHPFTISSAP
481EQKDTIWLHI RSQGQWTNRL YESFKASDPL GRGSKRLSRS VTMRKSQRSS KGSEILLEKH
541KFCNIKCYID GPYGTPTRRI FASEHAVLIG AGIGITPFAS ILQSIMYRHQ KRKHTCPSCQ
601HSWIEGVQDN MKLHKVDFIW INRDQRSFEW FVSLLTKLEM DQAEEAQYGR FLELHMYMTS
661ALGKNDMKAI GLQMALDLLA NKEKKDSITG LQTRTQPGRP DWSKVFQKVA AEKKGKVQVF
721FCGSPALAKV LKGHCEKFGF RFFQENF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

747 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.