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NOSTRIN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NOSTRIN Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NOSTRIN antibodies →
$504.00each
Catalog Number: LS-G120385-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NOSTRIN (NM_001039724), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BM247 homolog | ENOS traffic inducer | ENOS-trafficking inducer | NOSTRIN | DaIP2
Origin Species
Human
NCBI GenBank
NM_001039724

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRDPLTDCPY NKVYKNLKEF SQNGENFCKQ VTSVLQQRAN LEISYAKGLQ KLASKLSKAL
61QNTRKSCVSS AWAWASEGMK STADLHQKLG KAIELEAIKP TYQVLNVQEK KRKSLDNEVE
121KTANLVISNW NQQIKAKKKL MVSTKKHEAL FQLVESSKQS MTEKEKRKLL NKLTKSTEKL
181EKEDENYYQK NMAGYSTRLK WENTLENCYQ SILELEKERI QLLCNNLNQY SQHISLFGQT
241LTTCHTQIHC AISKIDIEKD IQAVMEETAI LSTENKSEFL LTDYFEEDPN SAMDKERRKS
301LLKPKLLRLQ RDIEKASKDK EGLERMLKTY SSTSSFSDAK SQKDTAALMD ENNLKLDLLE
361ANSYKLSSML AELEQRPQPS HPCSNSIFRW REKEHTHSYV KISRPFLMKR LENIVSKASS
421GGQSNPGSST PAPGAAQLSS RLCKALYSFQ ARQDDELNLE KGDIVIIHEK KEEGWWFGSL
481NGKKGHFPAA YVEELPSNAG NTATKA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

506 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.