Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NOS1AP / CAPON Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101249-100
Catalog Number: LS-G101249
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CAPON(NOS1AP) (NM_014697), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0464 | CAPON | NOS1AP
- Origin Species
- Human
- NCBI GenBank
- AF037070
- SwissProt
- O75052
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPSKTKYNLV DDGHDLRIPL HNEDAFQHGI CFEAKYVGSL DVPRPNSRVE IVAAMRRIRY61EFKAKNIKKK KVSIMVSVDG VKVILKKKKK KKEWTWDESK MLVMQDPIYR IFYVSHDSQD121LKIFSYIARD GASNIFRCNV FKSKKKSQAM RIVRTVGQAF EVCHKLSLQH TQQNADGQED181GESERNSNSS GDPGRQLTGA ERASTATAEE TDIDAVEVPL PGNDVLEFSR GVTDLDAVGK241EGGSHTGSKV SHPQEPMLTA SPRMLLPSSS SKPPGLGTET PLSTHHQMQL LQQLLQQQQQ301QTQVAVAQVH LLKDQLAAEA AARLEAQARV HQLLLQNKDM LQHISLLVKQ VQELELKLSG361QNAMGSQDSL LEITFRSGAL PVLCDPTTPK PEDLHSPPLG AGLADFAHPA GSPLGRRDCL421VKLECFRFLP PEDTPPPAQG EALLGGLELI KFRESGIASE YESNTDESEE RDSWSQEELP481RLLNVLQRQE LGDGLDDEIA VSGPSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
504 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.