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NOP9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NOP9 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G105136-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C14ORF21(NOP9) (NM_174913), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA2021 | NOP9 nucleolar protein | Nucleolar protein 9 | NOP9 | C14orf21
Origin Species
Human
SwissProt
Q86U38

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGQGPRSPHK VGRRFPAGGK RGRGAKGSGR PLPGRKRQPW PPPDGRSEPA PDSHPHLSPE
61ALGYFRRALS ALKEAPETGE ERDLMVHNIM KEVETQALAL STNRTGSEML QELLGFSPLK
121PLCRVWAALR SNLRTVACHR CGVHVLQSAL LQLPRLLGSA AEEEEEEEEE DGKDGPTETL
181EELVLGLAAE VCDDFLVYCG DTHGSFVVRT LLQVLGGTIL ESERARPRGS QSSEAQKTPA
241QECKPADFEV PETFLNRLQD LSSSFLKDIA VFITDKISSF CLQVALQVLH RKLPQFCAHL
301CNAVIGYLNT RGSSVDGSPL LLFLRDQTSS RLLEQVLLVL EPPRLQSLFE EHLQGQLQTL
361AAHPIANFPL QRLLDAVTTP ELLSPVFEEL SPVLEAVLAQ GHPGVVIALV GACRRVGAYQ
421AKVLQLLLEA FHCAEPSSRQ VACVPLFATL MAYEVYYGLT EEEGAVPAEH QVAMAAARAL
481GDVTVLGSLL LQHLLHFSTP GLVLRSLGAL TGPQLLSLAQ SPGRKLLLSL GSRTRS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

536 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.