LSBio
NOP10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NOP10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111636-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NOP10 (NM_018648), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKCB1 | Homolog of yeast Nop10p | NOLA3 | NOP10 | Nucleolar protein 10 | SnoRNP protein NOP10 | NOP10 ribonucleoprotein | NOP10P
Origin Species
Hu
NCBI GenBank
NM_018648
SwissProt
Q9NPE3

Protein

Expression System
HEK293
Fusion Tag
FLAG, Myc
Molecular Weight
7.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFLQYYLNEQ GDRVYTLKKF DPMGQQTCSA HPARFSPDDK YSRHRITIKK RFKVLMTQQP
61RPVL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

64 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconj
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.