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NOM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NOM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NOM1 antibodies →
$725.00each
Catalog Number: LS-G107371-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NOM1 (NM_138400), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C7orf3 | SGD1 homolog | NOM1 | SGD1
Origin Species
Human
NCBI GenBank
H97788
SwissProt
Q5C9Z4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAASRSAGEA GPGGSQGRVV RMKRRGGRGP RRGPAGGGEK ALKRLKLAVE EFVHATSEGE
61APGGCEGRGA PVSFRPGGRK SRKELRKEKR HLRKARRLQR TAGPEQGPGL GGRSGAEEAS
121GHRQDTEERA RPAPSRDPSP PRKPRPSRVK AKATAATAKT RPSAAATAAA RKRALLAANE
181EEDREIRKLE RCLGLNKRKK KDGSSSVPLS FARDGLDYIL GALESGKNSG LYDSSGEEEE
241DAGQTLPESD LESDSQDESE EEEEGDVEKE KKAQEAEAQS EDDDEDTEEE QGEEKEKGAQ
301EKRRGKRVRF AEDEEKSENS SEDGDITDKS LCGSGEKYIP PHVRQAEETV DFKKKEELER
361LKKHVKGLLN RLSEPNMASI SGQLEELYMA HSRKDMNDTL TSALMGACVT ASAMPSRLMM
421EHVLLVSILH HTVGIEVGAH FLEAVVRKFD AIYKYGSEGK ECDNLFTVIA HLYNFHVVQS
481LLIFDILKKL IGTFTEKDIE LILLMLKNVG FSLRKDDALS LKELITEAQT KASGAGSEFQ
541DQTRIRFMLE TMLALKNNDM RKIPGYDPEP VEKLRKLQRA LVRNAGSGSE TQLRVSWDSV
601LSAEQTGRWW IVGSAWSGAP MIDNSHHTHL QKQLVGTVSS KILELARKQR MNTDIRRNIF
661CTIMTSEDFL DAFEKLLKLG LKDQQEREII HVLMDCCLQE KTYNPFYAFL ASKFCEYERR
721FQMTFQFSIW DKFRDLENLP ATNFSNLVHL VAHLLKTKSL SLSILKVVEF SELDKPRVRF
781LRKVLSILLM ETEVEDLSLI FTRVSDNPKL GVLREGLKLF ISHFLLKNAQ AHRSADEANV
841LREKADLATK CLQGKASLRM
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

860 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.