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NOLC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NOLC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NOLC1 antibodies →
$504.00each
Catalog Number: LS-G100379-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NOLC1 (NM_004741), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Nucleolar protein p130 | NOLC1 | NS5ATP13 | Nucleolar 130 kDa protein | p130 | Nucleolar phosphoprotein p130 | KIAA0035 | NOPP130 | NOPP140
Origin Species
Human
NCBI GenBank
NM_004741
SwissProt
Q14978

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADAGIRRVV PSDLYPLVLG FLRDNQLSEV ANKFAKATGA TQQDANASSL LDIYSFWLNR
61SAKVPERKLQ ANGPVAKKAK KKASSSDSED SSEEEEEVQG PPAKKAAVPA KRVGLPPGKA
121AAKASESSSS EESSDDDDEE DQKKQPVQKG VKPQAKAAKA PPKKAKSSDS DSDSSSEDEP
181PKNQKPKITP VTVKAQTKAP PKPARAAPKI ANGKAASSSS SSSSSSSSDD SEEEKAAATP
241KKTVPKKQVV AKAPVKAATT PTRKSSSSED SSSDEEEEQK KPMKNKPGPY SSVPPPSAPP
301PKKSLGTQPP KKAVEKQQPV ESSEDSSDES DSSSEEEKKP PTKAVVSKAT TKPPPAKKAA
361ESSSDSSDSD SSEDDEAPSK PAGTTKNSSN KPAVTTKSPA VKPAAAPKQP VGGGQKLLTR
421KADSSSSEEE SSSSEEEKTK KMVATTKPKA TAKAALSLPA KQAPQGSRDS SSDSDSSSSE
481EEEEKTSKSA VKKKPQKVAG GAAPSKPASA KKGKAESSNS SSSDDSSEEE EEKLKGKGSP
541RPQAPKANGT SALTAQNGKA AKNSEEEEEE KKKAAVVVSK SGSLKKRKQN EAAKEAETPQ
601AKKIKLQTPN TFPKRKKGEK RASSPFRRVR EEEIEVDSRV ADNSFDAKRG AAGDWGERAN
661QVLKFTKGKS FRHEKTKKKR GSYRGGSISV QVNSIKFDSE SGPTRTRRLE QKLISEEDLA
721ANDILDYKDD DDKV

734 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.