Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NOL6 / NRAP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107071-100
Catalog Number: LS-G107071
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NOL6 (NM_139235), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA311H10.1 | Nucleolar protein 6 | NOL6 | UTP22
- Origin Species
- Human
- NCBI GenBank
- NM_022917
- SwissProt
- Q9H6R4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 77.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGPAPAGEQL RGATGEPEVM EPALEGTGKE GKKASSRKRT LAEPPAKGLL QPVKLSRAEL61YKEPTNEELN RLRETEILFH SSLLRLQVEE LLKEVRLSEK KKDRIDAFLR EVNQRVVRVP121SVPETELTDQ AWLPAGVRVP LHQVPYAVKG CFRFLPPAQV TVVGSYLLGT CIRPDINVDV181ALTMPREILQ DKDGLNQRYF RKRALYLAHL AHHLAQDPLF GSVCFSYTNG CHLKPSLLLR241PRGKDERLVT VRLHPCPPPD FFRPCRLLPT KNNVRSAWYR GQSPAGDGSP EPPTPRYNTW301VLQDTVLESH LQLLSTILSS AQGLKDGVAL LKVWLRQREL DKGQGGFTGF LVSMLVVFLV361STRKIHTTMS GYQVLRSVLQ FLATTDLTVN GISLCLSSDP SLPALADFHQ AFSVVFLDSS421GHLNLCADVT ASTYHQVQHE ARLSMMLLDS RADDGFHLLL MTPKPMIRAF DHVLHLRPLS481RLQAACHRLK LWPELQDNGG DYVSAALGPL TTLLEQGLGA RLNLLAHSRP PVPEWDISQD541PPKHKDSGTL TLGLLLRPEG LTSVLELGPE ADQPEAAKFR QFWGSRSELR RFQDGAIREA601VVWEAASMSQ KRLIPHQVVT HLLALHADIP ETCVHYVGGP LDALIQGLKE ASSTKGRMVM661SRGGELVMVP NVEAILEDFA VLGEGLVQTV EARSERWTVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
699 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.