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NOC4L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NOC4L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110023-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NOC4L (NM_024078), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NOC4L | NOC4 | NOC4 protein homolog | NOC4-like protein | UTP19 | NET49
Origin Species
Human
NCBI GenBank
NM_024078
SwissProt
Q9BVI4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEREPGAAGV RRALGRRLEA VLASRSEANA VFDILAVLQS EDQEEIQEAV RTCSRLFGAL
61LERGELFVGQ LPSEEMVMTG SQGATRKYKV WMRHRYHSCC NRLGELLGHP SFQVKELALS
121ALLKFVQLEG AHPLEKSKWE GNYLFPRELF KLVVGGLLSP EEDQSLLLSQ FREYLDYDDT
181RYHTMQAAVD AVARVTGQHP EVPPAFWNNA FTLLSAVSLP RREPTVSSFY VKRAELWDTW
241KVAHLKEHRR VFQAMWLSFL KHKLPLSLYK KVLLIVHDAI LPQLAQPTLM IDFLTRACDL
301GGALSLLALN GLFILIHKHN LEYPDFYRKL YGLLDPSVFH VKYRARFFHL ADLFLSSSHL
361PAYLVAAFAK RLARLALTAP PEALLMVLPF ICNLLRRHPA CRVLVHRPHG PELDADPYDP
421GEEDPAQSRA LESSLWELQA LQRHYHPEVS KAASVINQAL SMPEVSIAPL LELTAYEIFE
481RDLKKKGPEP VPLEFIPAQG LLGRPGELCA QHFTLS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

516 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.