Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NOB1 / NOB1P Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114170-100
Catalog Number: LS-G114170
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NOB1 (NM_014062), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ART-4 | MST158 | MSTP158 | NOB1 | PSMD8BP1 | NOB1P | Nin one binding protein | Protein ART-4 | PSMD8 binding protein 1 | RNA-binding protein NOB1
- Origin Species
- Human
- NCBI GenBank
- NM_014062
- SwissProt
- Q9ULX3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPVEHVVAD AGAFLRHAAL QDIGKNIYTI REVVTEIRDK ATRRRLAVLP YELRFKEPLP61EYVRLVTEFS KKTGDYPSLS ATDIQVLALT YQLEAEFVGV SHLKQEPQKV KVSSSIQHPE121TPLHISGFHL PYKPKPPQET EKGHSACEPE NLEFSSFMFW RNPLPNIDHE LQELLIDRGE181DVPSEEEEEE ENGFEDRKDD SDDDGGGWIT PSNIKQIQQE LEQCDVPEDV RVGCLTTDFA241MQNVLLQMGL HVLAVNGMLI REARSYILRC HGCFKTTSDM SRVFCSHCGN KTLKKVSVTV301SDDGTLHMHF SRNPKVLNPR GLRYSLPTPK GGKYAINPHL TEDQRFPQLR LSQKARQKTN361VFAPDYIAGV SPFVENDISS RSATLQVRDS TLGAGRRRLN PNASRKKFVK KRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
412 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.